采用專為低洗脫體積（低至6µL）設計的錐形凈化柱，可回收>50bp的DNA片段，只需30分鐘即可完成純化流程，是各種CUT&RUN靶點，包括組蛋白PTM和轉錄因子的理想選擇。
圖例：
 | Figure 1: CUT&RUN DNA fragment size distribution analysis 500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EpiCypher 13-2014) antibodies. Isolated DNA was used to prepare paired-end Illumina® sequencing libraries, which were analyzed by Agilent TapeStation® , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA + sequencing adapters). |
產品描述：
Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.
產品訂購
| 產品名稱 | 貨號 | 規格 |
| CUTANA™ DNA Purification Kit | 14-0050 | 50 Preps |
如需了解更多詳細信息或相關產品，請聯系EpiCypher中國授權代理商-欣博盛生物
聯系我們
傳真：86-0755-26755877
郵箱：market@neobioscience.com
地址：深圳市福田區上梅林梅華路105號多麗工業區科技樓0605室
掃一掃，關注我們
在線咨詢
電話咨詢
